HIF-1 alpha Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 175 publications

Catalog Number	Mfr. No.	Quantity
NB100134SS	Novus Biologicals NB100134SS	0.025 mL
NB100134	Novus Biologicals NB100134	0.1 mL

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus, Zebrafish samples. It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Gel Super Shift Assays, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing, Dual RNAscope ISH-IHC.

Specifications

• Antigen: HIF-1 alpha
• Dilution: Western Blot 1:500 - 1:1000, Simple Western 1:100, ELISA, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Immunoblotting, Gel Super Shift Assays 1:1 - 1:100, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635), Dual RNAscope ISH-IHC
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Gene Symbols: HIF1A
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Human, monkey, mouse, rat and Xenopus. Canine reactivity reported in scientific literature (PMID: 24140705) Mouse results are mixed.

• Concentration: 1.0 mg/mL
• Applications: ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Gel Shift, ChIP Assay, Immunoassay
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus species, Zebrafish
• Gene Accession No.: Q61221, O35800
• Gene ID (Entrez): 3091
• Immunogen: This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 93 kDa

For Research Use Only