HIF-1 alpha Antibody (H1alpha67), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB100123SS	Novus Biologicals NB100123SS	0.025 mL
NB100123	Novus Biologicals NB100123	0.1 mL

Description

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Ferret, Primate, Rabbit, Sheep samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunoblotting, Gel Super Shift Assays, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Concentration: 1.0 mg/mL
• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene Accession No.: Q16665
• Gene ID (Entrez): 3091
• Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 93 kDa

• Clone: H1alpha67
• Dilution: Western Blot 1:500 - 1:1000, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature, Immunohistochemistry 1:100 - 1:300, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10, Immunohistochemistry-Paraffin 1:100 - 1:300, Immunoblotting reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated reported in scientific literature (PMID 27991597), KnockDown Validated
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Avian, Bovine, Canine, Ferret, Primate, Sheep
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
• Gene Symbols: HIF1A
• Isotype: IgG2b
• Purification Method: Protein A purified
• Test Specificity: Recognizes human, mouse, rat, bovine, monkey, porcine, sheep, canine, bird and ferret HIF-1 alpha.

For Research Use Only