HIF-1 alpha Antibody (H1alpha67) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP280760

Catalog No. NB013833

Description

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Bovine, Canine, Feline, Ferret, Primate, Monkey, Rabbit, Sheep, Xenopus samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoassay, Immunoblotting, In vitro assay, Gel Super Shift Assays, Proximity Ligation Assay, Tissue Culture Substratum, Ligand Activation, CyTOF-ready, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot, Chromatin Immunoprecipitation 1 - 5 ug/IP. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 20042684), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:20 - 1:50, Immunohistochemistry-Frozen 1:20 - 1:50, Immunoassay reported in scientific literature (PMID 26147748), Immunoblotting reported in multiple pieces of scientific literature, In vitro assay reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature (PMID 22411794), Proximity Ligation Assay reported in scientific literature (PMID 27595394), Tissue Culture Substratum, Ligand Activation reported in scientific literature (PMID 26147748), Chromatin Immunoprecipitation (ChIP) 1-5 ug/IP, CyTOF-ready, Knockout Validated reported in scientific literature (PMID 26861754), Knockdown Validated
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Xenopus reactivity was reported in scientific literature (PMID: 18303027). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Canine reactivity reported in scientific literature (PMID: 24153191, 29974500). Feline reactivity reported in scientific literature (PMID: 30419801).
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2b

• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, In vitro Assay, KnockDown
• Clone: H1alpha67
• Conjugate: Unconjugated
• Formulation: PBS
• Gene Symbols: HIF1A
• Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cell Biology, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Cytokine Research, HIF Target Genes, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 3091
• Target Species: Human, Mouse, Rat, Pig, Bovine, Canine, Feline, Ferret, Primate, Monkey, Rabbit, Sheep, Xenopus species
• Form: Purified