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HIF-2 alpha/EPAS1 Antibody (ep190b) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NB013839 Shop All R&D Systems Products
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NB013839 0.1 mL
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Catalog No. NB013839 Supplier Novus Biologicals Supplier No. NBP280766
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Description

Mouse Monoclonal Antibody

HIF-2 alpha/EPAS1 Monoclonal specifically detects HIF-2 alpha/EPAS1 in Human, Mouse, Rat, Bovine, Hamster samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In vivo assay, Gel Supershift Assay, CyTOF-ready, Knockdown Validated.

Specifications

Antigen HIF-2 alpha/EPAS1
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, KnockDown
Classification Monoclonal
Clone ep190b
Concentration 1 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1 - 2 ug/mL, Simple Western 1:100, Flow Cytometry 1:400, ELISA : 1:100-1:2000, Immunohistochemistry 1:150 - 1:300, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:150 - 1:300, Immunohistochemistry-Frozen reported in scientific literature (PMID 24973414), Gel Super Shift Assays reported in scientific literature (PMID 17404621), In vivo assay reported in scientific literature (PMID 23857308), Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP), CyTOF-ready, Knockdown Validated
Formulation PBS
Gene Alias Basic-helix-loop-helix-PAS protein MOP2, BHLHE73, Class E basic helix-loop-helix protein 73, ECYT4, endothelial PAS domain protein 1, endothelial PAS domain-containing protein 1, EPAS1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-2 alpha, HIF2A, hif2a angiogenesis, HIF-2-alpha, HIF2-alpha, HLF, hypoxia-inducible factor 2 alpha, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, MOP2, PAS domain-containing protein 2, PASD2
Gene Symbols EPAS1
Host Species Mouse
Immunogen The immunogen recognized by this HIF-2 alpha/EPAS1 Antibody (ep190b) maps to a region between amino acids 535-631. [UniProt# Q99814]
Purification Method Protein G purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Angiogenesis, Apoptosis, Cancer, Cancer Stem Cells, Chromatin Research, HIF Target Genes, Hypoxia, Lipid and Metabolism, Transcription Factors and Regulators
Primary or Secondary Primary
Gene ID (Entrez) 2034
Test Specificity Ability to use HIF-2 alpha/EPAS1 Antibody (ep190b) in mouse is mixed with some positive and some negative results.
Target Species Human, Mouse, Rat, Bovine, Hamster
Content And Storage Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1
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