HIF1A 293T Cell Overexpression Lysate (Denatured), Abnova

Catalog No.	Quantity
89-009-079	100 μL

Catalog No. 89-009-079 Supplier Abnova Corporation Supplier No. H00003091T01

Description

Denatured HIF1A 293T Cell Overexpression Lysate

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)

• Transfected cell line: 293T
• Plasmid: pCMV-HIF1A full-length
• Host: Human
• Molecular weight: 80.96kDa
• Storage Buffer: 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Western Blotting

Specifications

• Cell Line: 293T
• For Use With (Application): Western Blot
• Description: HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
• Gene Accession No.: NM_181054
• Molecular Weight (g/mol): 82.7
• pH Range: 6.8
• Plasmid: pCMV-HIF1A full-length
• Quality Control Testing: Transient overexpression cell Lysate was tested with Anti-HIF1A antibody (H00003091-B01) by western blotting
• Quantity: 100 μL
• Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
• Host Species: Human
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.