Invitrogen™ HIF1AN Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA534852

Catalog No. PIPA534852

Description

Recommended positive controls: HCT116, MCF-7. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (90%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt].

Specifications

• Antigen: HIF1AN
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HIF1AN
• Gene Alias: 2310046M24Rik; A830014H24Rik; DKFZp762F1811; factor inhibiting HIF1; factor inhibiting HIF-1; FIH; FIH 1; FIH1; FIH-1; FLJ20615; FLJ22027; Hif1an; hypoxia inducible factor 1 alpha subunit inhibitor; hypoxia inducible factor 1 subunit alpha inhibitor; hypoxia inducible factor 1, alpha subunit inhibitor; hypoxia-inducible factor 1, alpha subunit inhibitor; hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 55662
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9NWT6
• Gene Symbols: HIF1AN
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HIF1AN. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov