Hif3 alphaRabbit anti-Mouse, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401435

Catalog No. 50-194-3846

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity-purified antibody is directed against mouse Hif3alpha protein. A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (also designated Arnt 1), EPAS-1 (also designated HIF-2 alpha) and HIF-3 alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.

Specifications

• Antigen: Hif3 alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HIF3A
• Gene Alias: Basic-helix-loop-helix-PAS protein MOP7; BHLHE17; Class E basic helix-loop-helix protein 17; HIF-3 alpha; HIF3 alpha 1; HIF3A; HIF-3A; HIF-3-alpha; HIF3-alpha; HIF3-alpha-1; hypoxia inducible factor 3 alpha; hypoxia inducible factor 3 alpha subunit; hypoxia inducible factor 3, alpha subunit; hypoxia inducible factor 3a; hypoxia inducible factor three alpha; hypoxia-inducible factor 3 alpha; Hypoxia-inducible factor 3-alpha; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; Mop7; Neonatal and embryonic PAS protein; Nepas; PAS domain-containing protein 7; PASD7
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 53417
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1.2 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: Q0VBL6
• Gene Symbols: HIF3A
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody