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  7. Gibco™ High-Intensity Perfusion CHO Medium

Gibco™ High-Intensity Perfusion CHO Medium

Catalog No. A4230203
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Catalog No. A4230203 Supplier Gibco™ Supplier No. A4230203
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Description

Gibco High-Intensity Perfusion (HIP) CHO Medium is an innovative, chemically defined, animal origin-free (AOF), cGMP medium.

Gibco High-Intensity Perfusion (HIP) CHO Medium is an innovative, chemically defined, animal origin-free (AOF), cGMP medium. This easy-to-use perfusion medium helps enable high titers in numerous CHO cell lines from the beginning to the end of the process regardless of the type of perfusion platform, all while using less medium.

Features of HIP CHO Medium include:

  • Tested with CHO-S, CHO-DG44, CHO-GS, and CHO-K1 cell lines
  • Supports high cell density of over 100 million cells/mL at 1 vessel volume/day (VVD) continuous perfusion
  • Demonstrated production of up to 1.7 g/L/day at 1 VVD continuous perfusion

Flexibility

HIP CHO Medium can be used at multiple concentrations to support seed train to production with one product. The flexible reconstitution of the medium allows dynamic tailoring of medium concentration to meet process and clone needs. Just as it can be used at multiple concentrations, HIP CHO Medium can be used in multiple perfusion processes: N-1 and seed train, intensified fed-batch, concentrated fed-batch, and continuous perfusion.In addition, HIP CHO Medium supports high cell densities at low medium exchange rates (as low as 1 VVD), which improves process efficiency.

HIP CHO Medium can help enable your move toward perfusion processes, which in turn can lead to a more compact and efficient workflow while improving productivity and quality.

Applications of HIP CHO Medium:

  • Suspension CHO cells
  • Selection system such as glutamine synthetase (GS) and dihydrofolate reductase (DHFR)
  • Passaging, seed train, and protein production applications
  • Optimized for use without feeds in perfusion application (other than glucose)

Specifications

Cell Line CHO-S, CHO-GS, CHO-K1, CHO-DG44
Concentration 34.92 g/L
Form Dry Powder
Product Type Perfusion CHO Medium
Sterility Non-sterile
With Additives Glucose
Without Additives No Glutamine
Manufacturing Quality cGMP
Quantity 100 L
Shipping Condition Dry Ice
Format Ropack bucket
Reconstituted Volume 100 L
Content And Storage Dry format medium. Store in the dark and dry at 2–8°C.
Culture Type Suspension Cell Culture
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