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His Tag Antibody, R&D Systems™
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Catalog No. MAB050R100
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100 μg
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MAB050R100 100 μg
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Mouse Monoclonal Antibody has been used in 2 publications

His Tag Monoclonal specifically detects His Tag in Multi-Species samples. It is validated for Western Blot, Flow Cytometry, Immunoaffinity Purification.

Specifications

Antigen His Tag
Applications Western Blot, Flow Cytometry, Affinity Purification
Classification Monoclonal
Clone AD1.1.10R
Conjugate Unconjugated
Dilution Western Blot 0.2 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunoaffinity Purification
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. with No Preservative
Gene Alias 6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, polyHistidine, Poly-histidine
Host Species Mouse
Immunogen His-tagged peptide
Purification Method Protein A or G purified from cell culture supernatant
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags located at the amino- or carboxyl- terminus.
Target Species Multi-species
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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