His-Tag Mouse anti-Tag, HRP, Clone: 1B7G5, Proteintech

Mouse Monoclonal Antibody

Supplier:  Proteintech Group Inc HRP66005100UL

Catalog No. 50-553-246

Description

This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) into the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.

Specifications

• Antigen: His-Tag
• Classification: Monoclonal
• Concentration: 0.4 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% thimerosal; pH 7.3
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: 1B7G5
• Conjugate: HRP
• Gene Alias: 6x His, 6xHis, His Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Tag