Cytiva HiScreen™ Sepharose™ Prepacked Fast Flow HIC Columns

Catalog No.	Stationary Phase
45-002-534	Butyl Sepharose
45-002-535	Butyl-S-Sepharose
45-002-538	Phenyl (low sub) Sepharose
45-002-536	Octyl Sepharose

Catalog No. 45-002-534 Supplier Cytiva Supplier No. 28926984

Description

Designed for method optimization and parameter screening

• Ready-to-use columns for convenient process development optimization
• Standard aliphatic/aromatic Hydrophobic Interaction Chromatography media for capture and intermediate purification
• Bed height of 10cm designed to allow method optimization and parameter screening
• Easily connected in series to achieve 20cm bed height
• Small bed volume of 4.7mL for fast result and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with same chromatography media using same linear fluid velocity

• Bed dimension: 7.7 × 100mm
• Matrix: 4% cross-linked agarose/6% cross-linked agarose
• pH stability working range (media): 3 to 13
• Average particle size: 90μm
• Material (column hardware and column tube): polypropylene
• Column I.D.: 7.7mm
• Pressure max.: 1.5 bar (22psi)
• Flow velocity: <240/450cm/hr.

Specifications

• Buffer: 1M NaOH, 30% Isopropanol, 70% Ethanol, 6M Guanidine Hydrochloride, 30% Aceto nitrile, 1mM HCl
• Product Type: HiScreen Butyl Sepharose 4 FF column
• Bed Volume: 4.7mL
• Diameter (English) Inner: 0.3 in.
• Diameter (Metric) Inner: 7.7mm
• Quantity: 1 x 4.7mL
• Height (English): 3.93 in.
• Height (Metric): 100mm
• Flow Rate: <240 cm/hr.
• Material: Polypropylene
• Ligand Type: Butyl
• Max. Pressure: 1.5 bar
• pH Range: 3 to 13
• Particle Size: 45 to 165μm
• Support Type: 4% Cross-linked Agarose
• Content And Storage: 4° to 30°C
• Stationary Phase: Butyl Sepharose
• Column Type: Ion Exchange Column
• For Use With (Application): Convenient process development optimization parameter screening