Thermo Scientific HisPur Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Manufacturer: Thermo Scientific 88222
Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.
HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.
High capacity bind up to 60mg of 6xHis-tagged protein per milliliter of resin
Versatile purify proteins using native or denaturing conditions
Compatible use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Flexible available in multiple formats including bulk resin, spin columns and chromatography cartridges
Cost-effective reuse the same batch of resin at least five times
Easy to use pre-formulated buffers available for kit formats
- Resin Slurries crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
- Spin Columns 0.2mL (microcentrifuge), 1mL, 3mL columns
- Purification Kits complete kits in all three column sizes
- Chromatography Cartridges compatible with automated liquid chromatography systems and with syringe processing
- Bulk and Custom let us know your needs for bulk HisPur Ni-NTA Resin
Kits contain HisPur Ni-NTA Columns
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|Agarose Resin, Affinity|
|HisPur Ni-NTA Resin|
|Store at 4°C|
|Divalent Nickel, Metal Chelate|