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Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL PROMO
Description
The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.
HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.
Highlights:
High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
Versatile – purify proteins using native or denaturing conditions
Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
Cost-effective – reuse the same batch of resin at least five times
Easy to use – pre-formulated buffers available for kit formats
Available Formats:
- Resin Slurries – crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
- Spin Columns – 0.2 mL (microcentrifuge), 1 mL, 3 mL columns
- Purification Kits – complete kits in all three column sizes
- Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
- Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin
Includes:
Kits contain HisPur Ni-NTA Columns
Specifications
Specifications
Capacity (Metric) | 1 mL/Column |
Content And Storage | Sufficient For: Purifying up to 180 mg of His-tagged protein per column, HisPur Ni-NTA Spin Columns, 3 mL, 5 Columns, Phosphate-buffered Saline (10X), 30 mL, Imidazole (2M), 25 mL, Column Plugs, 5 Plugs, Store at 4°C. |
Product Line | HisPur™ |
Quantity | 5 Column Kit |
Target | His-Tagged Protein |
Type | Spin Column Kit |
Stationary Phase | Divalent Nickel, Metal Chelate |
Sufficient For | 180 mg of His-Tagged Protein per Column |
Format | Spin Column |
Column Type | Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.
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