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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose, 10 mL PROMO
Description
HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.
Highlights:
High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour
High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates
Versatile – can be used to purify proteins under native and denaturing conditions
Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour
Compatible – maintains function in a wide variety of chemicals and pH conditions
Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Medium- to large-scale FPLC purification of polyhistidine tagged proteins
Specifications
Specifications
Capacity (Metric) | 20 mg His-Tagged GFP Binding per mL Resin |
Content And Storage | Store at 4°C |
Form | Liquid Suspension |
Product Line | HisPur™ |
Quantity | 10 mL |
Target | His-Tagged Protein |
Type | Agarose |
Stationary Phase | Divalent Nickel, Metal Chelate |
Format | Bottle |
Column Type | Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.
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