Histone H1 Antibody (21NC85), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB10065220

Catalog No. NB10065220

Description

Histone H1 Monoclonal specifically detects Histone H1 in Human, Rat, Porcine samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Histone H1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Alias: H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 3024
• Target Species: Human, Rat, Pig
• Isotype: IgG2a

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 21NC85
• Dilution: Western Blot 1:100-1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:20-1:30, Immunohistochemistry-Paraffin 1:500, Immunohistochemistry-Frozen 1:20-1:50
• Gene Symbols: H1-1
• Immunogen: The exact immunogen is proprietary, but is composed of lysed nuclei of myeloid leukemia biopsy cells.
• Quantity: 0.05 mL
• Primary or Secondary: Primary
• Test Specificity: This antibody recognises an antigen found in the nucleus. Antibody also preferentially reacts with histone H1/ DNA nucleosome as shown by ELISA and western blotting. In WB using non-reduced samples it recognises primarily a 30-32kDa histone H1 band, but other bands are present as well. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells. The epitope has not been mapped.
• Content And Storage: Store at -20C. Avoid freeze-thaw cycles.

For Research Use Only