Invitrogen™ Histone H3.3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA522388

Catalog No. PIPA522388

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Specifications

• Antigen: Histone H3.3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: H3-3A
• Gene Alias: 9430068D06Rik; CG5825; CG5825-PA; CG5825-PC; CG5825-PD; CG8989; dH3.3A; Dmel\CG5825; Dmel_CG5825; family 3B (H3.3B); H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3.3; H3.3 histone A; H3.3 histone B; H3.3A; H3.3A/B; H3.3B; H3.4; H3.A; H3.B; H3/g; H3-3A; H3-3B; H3B; H3F3; H3F3A; H3F3A protein; H3F3B; H3FT; H3-IX; H3L-like histone; H3t; H3-X; His3.3; His3.3A; His-3.3A; His3.3A-PA; His3.3A-PC; His3.3A-PD; His3.3B; histone; Histone cluster 3; Histone H3; histone H3 class II; Histone H3.3; histone H3.3A; Histone H3.3B; histone H3.3-like protein; MGC126886; MGC126888; PH3; PP781; similar to H3 histone, family 3A; unnamed protein product
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3020, 3021
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.16 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P84243
• Gene Symbols: H3-3A, H3-3B
• Immunogen: Full length human Histone H3.3B Recombinant protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG