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Histone H3 ( (K4) Rabbit, Clone: RM186, Abnova™

Rabbit monoclonal antibody raised against synthetic peptide of human Histone H3 (K4).

Manufacturer:  Abnova Corporation MAB12789

Catalog No. 89-934-250

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]



Histone H3 ( (K4)
Rabbit monoclonal antibody raised against synthetic peptide of human Histone H3 (K4).
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
100 ug
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
This antibody recognizes Histone H3 that is unmodified at Lys 4 but does not recognize acetylated or methylated Lys 4. The antibody binding specificity allows for modifications of Arg 2, Thr 3, and/or other modifications in Histone H3.
ELISA, Western Blot
ELISA (0.5 ug/mL-1 ug/mL) Western Blot (1 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
A synthetic peptide corresponding to residues surrounding K4 at N-terminus region of Histone H3.
Protein A purification
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