Histone H3 (trimethyl K27) Rabbit, Clone: RM175, Abnova™

Rabbit monoclonal antibody raised against synthetic trimethyl peptide of human histone H3 (trimethyl K27).

Supplier:  Abnova Corporation MAB12846

Catalog No. 89-947-507

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Specifications

• Antigen: Histone H3 (trimethyl K27)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: ELISA (0.5-1 ug/mL) Immunohistochemistry (0.5-2 ug/mL) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
• Gene Alias: H3/A/H3FA
• Host Species: Rabbit
• Purification Method: Protein A affinity purification
• Regulatory Status: RUO
• Gene ID (Entrez): 8350
• Target Species: Human
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (PFA fixed), Western Blot
• Clone: RM175
• Description: Rabbit monoclonal antibody raised against synthetic trimethyl peptide of human histone H3 (trimethyl K27).
• Formulation: In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
• Gene Symbols: HIST1H3A
• Immunogen: A synthetic trimethyl peptide corresponding to residues surrounding K27 of human histone H3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody reacts to histone H3 trimethylated at Lysine 27. No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 or dimethylated Lysine 27, or other methylations in histone H3.
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG