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Histone H3 (trimethyl-K9/phospho S10) Rabbit, Clone: RM162, Abnova™

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89-934-225 100 μg
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Catalog No. 89-934-225 Supplier Abnova Corporation Supplier No. MAB12797
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Description

Rabbit monoclonal antibody raised against synthetic trimethyl-phospho-peptide of human Histone H3 (trimethyl-K9/phospho S10).

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Specifications

Antigen Histone H3 (trimethyl-K9/phospho S10)
Applications ELISA, Western Blot
Classification Monoclonal
Clone RM162
Conjugate Unconjugated
Description Rabbit monoclonal antibody raised against synthetic trimethyl-phospho-peptide of human Histone H3 (trimethyl-K9/phospho S10).
Dilution ELISA (0.01 ug/mL-0.5 ug/mL) Western Blot (0.01 ug/mL-1 ug/mL) The optimal working dilution should be determined by the end user.
Formulation In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Gene Alias H3/A/H3FA
Gene Symbols HIST1H3A
Host Species Rabbit
Immunogen A synthetic trimethyl-phospho-peptide corresponding to residues surrounding trimethyl K9 and phospho S10 of Histone H3.
Purification Method Protein A purification
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 8350
Test Specificity This antibody reacts to Histone H3 only when modified AT both trimethyl lysine 9 and phospho serine 10.
Target Species Human
Content And Storage Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Form Liquid
Isotype IgG
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