Cytiva™ HiTrap™ Chromatography Columns with MabSelect™ VL Protein L Resin

Protein L affinity chromatography columns for capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.

Catalog Number	Mfr. No.	Bed Volume	Diameter (Metric) Inner	Flow Rate	Quantity
01-183-307	Cytiva™ 17542051	1 mL	7 mm	<4 mL/min.	1 x 1 mL
01-183-308	Cytiva™ 17542052	1 mL	7 mm	<4 mL/min.	5 x 1 mL
01-183-309	Cytiva™ 17542053	5 mL	16 mm	<20 mL/min.	1 x 5 mL
01-183-310	Cytiva™ 17542054	5 mL	16 mm	<20 mL/min.	5 x 5 mL

Description

• Redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability
• High dynamic binding capacity for efficient capture of target molecules
• Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents
• Provides good resolution for product-related impurities in the capture of bispecific antibodies

As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.

Specifications

• Height (English): 1 in.
• Max. Pressure: 5 bar
• For Use With (Application): Antibody Affinity Chromatography

• Height (Metric): 25 mm
• Column Type: Affinity Column