Cytiva HiTrap™ IMAC Columns

Catalog No.	Quantity	Diameter (English) Inner	Particle Size
45-000-164	5 x 5 mL	0.62 in.	34 μm
45-000-163	5 x 1 mL	0.27 in.	34 μm
45-000-167	5 x 1 mL	0.27 in.	90 μm
45-000-168	5 x 5 mL	0.62 in.	90 μm

Catalog No. 45-000-164

Supplier: Cytiva 17092005

Description

Includes

Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

For optimizing purification of histidine-tagged proteins when Ni ⁺² is not the best choice of metal ion

• Conveniently charge with your metal of choice
• Can be used with a syringe, peristaltic pump, or liquid chromatography system
• Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
• High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
• Stable in the presence of all commonly used buffers
• Available in 1mL and 5mL sizes

Specifications

• Bed Volume: 5 mL
• Diameter (English) Inner: 0.62 in.
• Flow Rate: <4 mL/min.
• Diameter (Metric) Inner: 16 mm
• Kit Contents: Instructions, plus a variety of connecto rs for syringe, peristaltic pump and chromato graphy systems
• For Use With (Equipment): Syringe, peristaltic pump or liquid chromato graphy system
• Material: Polypropylene
• Max. Pressure: 42 psi
• pH Range: 3 to 12
• Particle Size: 34 μm
• Product Type: HiTrap IMAC HP Column
• Content And Storage: 4°C to 30°C
• Quantity: 5 x 5 mL
• For Use With (Application): For optimizing purification of His-tagged Proteins