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Cytiva HiTrap™ rProtein A FF Columns

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

$495.00 - $4104.00


Binding Capacity ≈50 mg Human IgG/mL
Content And Storage 4°C to 30°C, 20% Ethanol
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
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Products 4
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity & Availability  
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5 x 1 mL <4 mL/min.
Pack of 5 for $1,014.00
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2 x 1 mL <4 mL/min.
Pack of 2 for $495.00
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5 x 5 mL <20 mL/min.
Pack of 5 for $4,104.00
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1 x 5 mL <20 mL/min.
Each for $994.00
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  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
  • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
  • Average particle size: 90μm
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Available as 1mL and 5mL columns
  • Ligand: Recombinant protein A (E.coli)
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: ∽50mg human IgG/mL
  • pH stability working range: 3 to 10

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

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1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.



≈50 mg Human IgG/mL
4°C to 30°C, 20% Ethanol
Chromatography System, Manual
For syringe, peristaltic pump or a chromatography system
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