Invitrogen™ HK2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529326

Catalog No. PIPA529326

Description

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, Mouse brain. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (86%), Pig (96%), Chicken (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Specifications

• Antigen: HK2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Hk2
• Gene Alias: AI642394; DKFZp686M1669; Hexokinase; hexokinase 2; hexokinase II; hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Hk2; HKII; HXK2; muscle form hexokinase; OTTHUMP00000202738
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100009677, 15277, 25059, 3099
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.63 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: A2PYL6, O08528, P27881, P52789
• Gene Symbols: Hk2
• Immunogen: Recombinant fragment corresponding to a region within amino acids 53 and 342 of Human Hexokinase II.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Horse
• Product Type: Antibody
• Isotype: IgG