Invitrogen™ HLA-A2 Monoclonal Antibody (BB7.2)

Catalog No.	Quantity
MA180117	200 μg

Catalog No. MA180117 Supplier Invitrogen™ Supplier No. MA180117

Description

Mouse Monoclonal Antibody

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. A suggested positive control for immunohistochemical applications is tonsil. Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen.

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-A2
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Classification: Monoclonal
• Clone: BB7.2
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene: HLA-A
• Gene Accession No.: P04439
• Gene Alias: A29 protein; antigen presenting molecule; Aw-33; Aw-74; DAQB-90C11.16; FLJ26655; HLA A; HLA class I antigen; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA locus; HLAA; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-A11; HLA-A33; HLA-Aw33.1; HLA-DQB1; HLA-DRB1; Human leukocyte antigen A; leucocyte antigen A; leukocyte antigen class I-A; major histocompatibility complex, class I, A; MCH class I antigen; MHC 1; MHC class 1 antigen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen heavy chain; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen null protein; MHC class I antigene; MHC class II antigen; MHC classI antigen; MHC1; truncated MHC class I antigen
• Gene Symbols: HLA-A
• Host Species: Mouse
• Immunogen: Papain solubilised HLA-A2.
• Purification Method: Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3105
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov