HLA-C Mouse anti-Human, PE, Clone: DT-9 (also known as DT9), BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 566372

Catalog No. BDB566372

Description

The DT-9 monoclonal antibody specifically recognizes Human Leukocyte Antigen (HLA-C), a polymorphic major histocompatibility complex (MHC) class I antigen. HLA-C is a heterodimer comprised of the HLA-C alpha chain, a ñ45 kDa type I transmembrane glycoprotein, and a ñ12 kDa Beta-2 (β2)-microglobulin light chain. HLA-C is expressed on nearly all cells, and plays a role in the antigen-specific, MHC-restricted presentation of small peptides to CD8+ T cells in the generation of immunity or tolerance. HLA-C may also bind to regulatory MHC class I antigen-selective receptors expressed by CD8+ T cells and natural killer (NK) cells. The DT-9 antibody also recognizes some rare HLA-A and HLA-B allotypes and the nonclassical HLA-E molecule.

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Specifications

• Antigen: HLA-C
• Conjugate: PE
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse IgG_2b, κ
• Quantity: 100 Tests
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Clone: DT-9 (also known as DT9)
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Gene Alias: HLAC, HLC-C, HLA-JY3, MHC class I antigen C, PSORS1, HLA-E
• Immunogen: Purified Human Tamarin MHC Class I Molecules
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human