Invitrogen™ HLA-C Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA587657

Catalog No. PIPA587657

Description

Positive Samples: LO2, HL-60, BT-474, Jurkat, Mouse liver; Cellular Location: Membrane, Single-pass type I membrane protein Immunogen sequence: CSHSMKYFFT SVSRPGRGEP RFISVGYVDD TQFVRFDSDA ASPRGEPRAP WVEQEGPEYW DRETQKYNRQ AQTDRVSLRN LRGYYNQSEA GSHTLQWMCG CDLGPDGRLL RGYDQYAYDG KDYIALNEDL RSWTAADTAA QITQRKWEAA REAEERRAYL EGTCVEWLRR YLENGKESLQ RAEHPKTHVT HHPVSDHEAT LRCWALGFYP AEITLTWQWD GEDQTQDTEL VETRPAGDGT FQKWAAVMVP SGEEQRYTCH VQHEGLPEPL TLRWEPSSQP TIPI.

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-C
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HLA-C
• Gene Alias: alpha chain of MHC class II; D6S204; HLA C; HLA class I histocompatibility antigen C alpha chain; HLA class I histocompatibility antigen Cw-1 alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA class I histocompatibility antigen, Cw-1 alpha chain; HLA class I histocompatibility antigen, Cw-15 alpha chain; HLA class I histocompatibility antigen, Cw-18 alpha chain; HLAC; HLA-C; HLA-C alpha chain; HLA-C alpha chain (HLA-Cw*15v); HLA-C alpha chain for Cw*18GB; HLA-C alpha-chain; HLA-C antigen; HLA-Cw; HLA-Cw*04GB; HLA-Cw*1702; HLA-Cw*18GB; HLA-Cw-1503; HLA-JY3; HLC-C; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; leukocyte antigen C; major histocompatibility antigen HLA-C; major histocompatibility complex, class I, C; MHC; MHC clas I antigen; MHC class 1 antigen; MHC Class I antigen; MHC class I antigen Cw*15; MHC class I antigen Cw*18; MHC class I antigen heavy chain HLA-C; MHC class I histocompatibility antigen; MHC class I molecule; MHC class II antigen; PSORS1; surface antigen; truncated MHC class I antigen
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3107
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 1.93 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P10321
• Gene Symbols: HLA-C
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-308 of human HLA-C (P30499).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG