MilliporeSigma™ HLA Class I Antigens, Mouse, Unlabeled, Clone: W6/32,

Catalog No.	Quantity	Conjugate
MABN1783MI	100 μL	Unconjugated

Catalog No. MABN1783MI Supplier MilliporeSigma™ Supplier No. MABN1783

Description

Mouse Monoclonal Antibody

Major histocompatibility complexes (MHCs), also called human leukocyte antigens (HLAs) in human, are cell surface molecules that play a major part of the immune system in all vertebrates by determining histocompatibility. The main function of MHCs is to bind and present peptide fragments derived from pathogens on the surface of antigen presenting cells. MHCs are divided into three classes, class I MHCs are heterodimers composed of a transmembrane alpha chain and a common extracellular beta subunit. The alpha chain contains three domains (alpha1, alpha2, and alpha3), with the alpha1 domain mediating interaction with the beta2 subunit. The alpha1 and alpha2 domains form the antigen-binding groove. Class II MHCs are heterodimers of two single transmembrane chains, alpha and beta, each contains two domains, with the N-terminal alpha1 and beta1 domain from each chain forming the antigen-binding groove. Class III molecules include several secreted proteins with immune functions, including components of the complement system, cytokines, and heat shock proteins. Each human cell expresses six MHC class I alleles (one HLA-A, -B, and -C allele from each parent). Classical MHCs present antigens to the TCRs of CD8+ T lymphocytes. Nonclassical molecules (class IB MHC) exhibit limited polymorphism, expression patterns, and presented antigens. This group is subdivided into a group encoded within MHC loci (e.g. HLA-E, -F, -G) and those not (e.g. ULBPs, Rae1, and H60).

Specifications

• Antigen: HLA Class I Antigens
• Applications: Affinity Purification, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Neutralization
• Classification: Monoclonal
• Clone: W6/32
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
• Gene: HLA-A, HLAA, HLA-B, HLAB, HLA-C, HLAC, HLA-E, HLAE
• Gene Accession No.: P30443;Q96DW9;O19617;P13747
• Host Species: Mouse
• Immunogen: Membrane from human tonsil lymphocyte preparations (Barnstable, C.J., et al. (1978). Cell. 14(1):9-20).
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001229687;CAD30340;NP_001229971;NP_005507
• Test Specificity: Clone W6/32 targets HLA classe I heavy chains (HLA-A, -B, -C, -E, and possibly -F) in a light chain-dependent manner, although clone W6/32 does not exhibit affinity toward isolated light chain (β2-microglobulin or β2-m). A tight interaction between β2-m residue 89 and some residues within the heavy chain second domain might be responsible for the formation of the W6/32 antigenic determinant which, however, is only expressed (or exposed) when β2-m cannot covalently bind to the heavy chain Cys121 (Kahn-Perles, B., et al. (1987). J. Immunol. 138(7).2190-2196). Cell surface MHC I molecules reconstituted with allele-specific peptides and recombinant human β2-m that contains a methionine at the N-terminus is not recognized by W6/32 (Shields, M.J., and Ribaudo, R.K. (1998). Tissue Antigens. 51(5):567-570). Clone W6/32 is reported to bind murine, rat, rabbit and guinea pig HLA class I heavy chains only when they are associated with bovine β2-m, but not with their respective autologous β2-m (Kahn-Perles, B., et al. (1987). J. Immunol. 138(7).2190-2196).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG2a

For Research Use Only. Not for use in diagnostic procedures.