Invitrogen™ HLA-DOA Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5109820

Catalog No. PIPA5109820

Description

Immunogen sequence: TKADHMGSYG PAFYQSYGAS GQFTHEFDEE QLFSVDLKKS EAVWRLPEFG DFARFDPQGG LAGIAAIKAH LDILVERSNR SRAINVPPRV TVLPKSRVEL GQPNILICIV DNIFPPVINI TWLRNGQTVT EGVAQTSFYS QPDHLFRKFH YLPFVPSAED VYDCQVEHWG LDAPLLRHWE LQVPIPPPDA ME.

Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigen-presenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-DOA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HLA-DOA
• Gene Alias: HLA class II histocompatibility antigen, DO alpha chain; HLA-D0-alpha; HLA-DNA; HLA-DOA; HLADZ; HLA-DZA; lymphocyte antigen; major histocompatibility complex, class II, DN alpha; major histocompatibility complex, class II, DO alpha; MHC class II antigen DOA; MHC DN-alpha; MHC DZ alpha
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3111
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 2.14 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: P06340
• Gene Symbols: HLA-DOA
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human HLA-DOA (NP_002110.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG