HLA-DP Mouse anti-Human, Clone: SPM421, Abnova™

Mouse monoclonal antibody raised against native human HLA-DP.

Supplier:  Abnova Corporation MAB13075

Catalog No. 89-934-262

Description

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq]

Specifications

• Antigen: HLA-DP
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
• Gene Alias: DPB1/HLA-DP1B
• Host Species: Mouse
• Purification Method: Protein A/G purification
• Regulatory Status: RUO
• Gene ID (Entrez): 3115
• Target Species: Human
• Form: Liquid

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed)
• Clone: SPM421
• Description: Mouse monoclonal antibody raised against native human HLA-DP.
• Formulation: In 10mM PBS (0.05% BSA, 0.05% sodium azide).
• Gene Symbols: HLA-DPB1
• Immunogen: Human non-T/non-B-cell-type acute lymphoblastic leukemia REH6 cell line.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
• Content And Storage: Store at 4°C.
• Isotype: IgG2b κ