HLA-DQ Mouse anti-Human, Clone: SPM422, Abnova™

Catalog No.	Quantity
89-934-266	100 μg

Catalog No. 89-934-266 Supplier Abnova Corporation Supplier No. MAB13077

Description

Mouse monoclonal antibody raised against native human HLA-DQ.

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

Specifications

• Antigen: HLA-DQ
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed)
• Classification: Monoclonal
• Clone: SPM422
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against native human HLA-DQ.
• Dilution: Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
• Formulation: In 10mM PBS (0.05% BSA, 0.05% sodium azide).
• Gene Alias: CD/CELIAC1/DQ-A1/FLJ27088/FLJ27328/GSE/HLA-DQA/MGC149527
• Gene Symbols: HLA-DQA1
• Host Species: Mouse
• Immunogen: T4-positive CTL clone HG-38.
• Purification Method: Protein A/G purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3117/3118/3119
• Test Specificity: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This monoclonal antibody strongly blocks cytotoxicity activity of T4⁺ cytotoxic T cell clones.
• Target Species: Human
• Content And Storage: Store at 4°C.
• Form: Liquid
• Isotype: IgG2a κ