HLA-DRB1 Mouse anti-Human, Clone: LN-3, Abnova™

Catalog No.	Quantity
89-934-275	100 μg

Catalog No. 89-934-275 Supplier Abnova Corporation Supplier No. MAB13306

Description

Mouse monoclonal antibody raised against native human HLA-DRB1.

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Specifications

• Antigen: HLA-DRB1
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed), Western Blot
• Classification: Monoclonal
• Clone: LN-3
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against native human HLA-DRB1.
• Dilution: Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be d
• Formulation: In 10mM PBS.
• Gene Alias: DRB1/FLJ76359/HLA-DR1B/HLA-DRB/HLA-DRB1*/SS1
• Gene Symbols: HLA-DRB1
• Host Species: Mouse
• Immunogen: Activated human peripheral blood mononuclear cells.
• Purification Method: Protein A/G purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3123
• Test Specificity: This monoclonal antibody does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4⁺ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B cell lymphoma.
• Target Species: Human
• Content And Storage: Store at -20 to -80°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgG2b κ