Invitrogen™ HLA-DRB5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578004

Catalog No. PIPA578004

Description

Positive Control: Raji, Raji membrane fraction extract Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: HLA-DRB5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HLA-DRB5
• Gene Alias: DR beta-5; DR2-beta-2; DR9; DR-9; DRB1-9 beta chain; dw2; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; hla-dr5; HLA-DRB; HLA-DRB5; human leucocyte antigen DRB5; leukocyte antigen class II; major histocompatibility complex, class II, DR beta 5; MHC class I antigen; MHC class II antigen DRB1*9; MHC class II antigen DRB5; MHC class II HLA beta chain; MHC HLA-DR; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3127
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.56 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q30154
• Gene Symbols: HLA-DRB5
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human HLA-DRB5. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG