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HLA-E Mouse anti-Human, PE, Clone: 3D12HLA-E, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12995342

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Catalog No. 50-113-15


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Description

Description

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous. Applications Reported: This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D12HLA-E antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Specifications

Specifications

HLA-E
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P13747
DAQB-90C11.16, FLJ26655, HLAA
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
3D12HLA-E
PE
HLA-E
Liquid
HLA-E
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3133
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