hLYVE-1, Mouse anti-Human, Clone: 8C, Millipore Sigma™

Catalog No.	Quantity
MABS192925	25 μg

Catalog No. MABS192925 Supplier MilliporeSigma Supplier No. MABS192925UG

Description

Mouse Monoclonal Antibody

Anti-hLYVE-1, clone 8C, Cat. No. MABS1929, is a mouse monoclonal antibody that detects Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE1) and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

Lymphatic vessel endothelial hyaluronic acid receptor 1 (UniProt: Q9Y5Y7; LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1,Extracellular link domain-containing protein 1,Hyaluronic acid receptor) is encoded by the LYVE1 (also known as CRSBP1, HAR, XLKD1,UNQ230/PRO263) gene (Gene ID: 10894) in human. LYVE-1 is a disulfide-linked, homodimeric, single-pass type I membrane protein that undergoes ligand-dependent internalization and recycling at the cell surface.  It serves as a ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane.  It is mainly expressed in endothelial cells lining lymphatic vessels. It is expressed on both the luminal and basolateral surfaces of lymphatic endothelium. It is reported to mediate the uptake of intermediate and high molecular weight hyaluronan (HA) and serves as a low affinity receptor for HA-induced lymphoangiogenesis. It also controls the entry and/or migration of leukocytes in lymphatic vessels. The capacity of LYVE-1 to bind HA is based on a receptor distribution and organization of the glycosaminoglycans. (Ref.: Lawrence, W et al. (2016). J Biol Chem. 291(15): 8014 8030).

Specifications

• Antigen: hLYVE-1
• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Clone: 8C
• Conjugate: Unconjugated
• Dilution: Immunocytochemistry Analysis: A representative lot detected hLYVE-1 in Immunocytochemistry applications (Lawrance, W., et. al. (2016). J Biol Chem. hLYVE-1; Banerji, S., et. al. (1999). J Cell Biol. 144(4):789-801). Western Blotting Analysis: A representative lot detected hLYVE-1 in Western Blotting applications (Banerji, S., et. al. (1999). J Cell Biol. 144(4):789-801; Banerji, S., et. al. (2016). J Biol Chem. 291(48):25004-25018).Flow Cytometry Analysis: A representative lot detected hLYVE-1 in Flow Cytometry applications (Lawrance, W., et. al. (2016). J Biol Chem. hLYVE-1; Banerji, S., et. al. (2016). J Biol Chem. 291(48):25004-25018).
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Lymphatic vessel endothelial hyaluronic acid receptor 1;Cell surface retention sequence-binding protein 1;CRSBP-1;Extracellular link domain-containing protein 1;Hyaluronic acid receptor
• Gene Symbols: LYVE1;CRSBP1;HAR;XLKD1;UNQ230/PRO263
• Host Species: Mouse
• Immunogen: A recombinant fragment corresponding to the ectodomain (1-232) region of human LYVE-1.
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Clone 8C detects Lymphatic vessel endothelial hyaluronic acid receptor 1. It targets an epitope within the ectodomain region.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1