Invitrogen™ HMCES Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA560876

Catalog No. PIPA560876

Description

Immunogen sequence: WRLLTMAGIF DCWEPPEGGD VLYSYTIITV DSCKGLSDIH HRMPAILDGE EAVSKWLDFG EVSTQEALKL IHPTENITFH AVSSVVNNS Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 88%.

Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877, PubMed:31235915, PubMed:31235913). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (PubMed:30554877, PubMed:31235913). The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877). Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction (PubMed:31235915, PubMed:31806351). Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine. [UniProt].

Specifications

• Antigen: HMCES
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HMCES
• Gene Alias: 5-hydroxymethylcytosine (hmC) binding, ES cell specific; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine binding, ES cell specific; 8430410A17Rik; Abasic site processing protein HMCES; abasic site processing protein HMCES; embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; C20H3orf37; C22H3orf37; C3orf37; C85376; DC12; Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; HMCES; Peptidase HMCES; Putative endonuclease HMCES; putative peptidase SRAPD1; SRAP domain-containing protein 1; Srap1; Srapd1; UPF0361 protein C3orf37; UPF0361 protein C3orf37 homolog
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56941
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q96FZ2
• Gene Symbols: HMCES
• Immunogen: Recombinant protein corresponding to Human HMCES. Recombinant protein control fragment (Product #RP-100261).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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