Invitrogen™ HMGB1 Recombinant Rabbit Monoclonal Antibody (1A1)

Catalog No.	Quantity
PIMA538353	100 μL

Catalog No. PIMA538353 Supplier Invitrogen™ Supplier No. MA538353

Description

Rabbit Recombinant Monoclonal Antibody

This antibody has been tested in direct-ELISA.

High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Specifications

• Antigen: HMGB1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: 1A1
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA, 50% glycerol, 150mM NaCl and 0.02% sodium azide; pH 7.4
• Gene: Hmgb1
• Gene Accession No.: P09429, P63158, P63159
• Gene Alias: Ac2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product
• Gene Symbols: Hmgb1
• Host Species: Rabbit
• Immunogen: A synthesized peptide derived from human HMGB1 (aa 150-200).
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15289, 25459, 3146
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C or -80°C if preferred
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG