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HMGB1 Mouse anti-Human, Mouse, Rat, Clone: GT427, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA527755

Catalog No. PIMA527755


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Description

Description

Positive Control: 293T, SK-N-SH, IMR32, SK-N-AS, Mouse brain, Rat brain Predicted Reactivity: Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.
Specifications

Specifications

HMGB1
Monoclonal
1 mg/ml
PBS with no preservative
P09429, P63158, P63159
Hmgb1
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
Protein G
RUO
Antibody
Monoclonal
Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
GT427
Unconjugated
HMGB1
Liquid
Mouse
IgG1
100 μl
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
15289, 25459, 3146
Documents
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