Invitrogen™ HMGB1 Polyclonal Antibody

Catalog No.	Quantity
PIPA527378	100 μL

Catalog No. PIPA527378 Supplier Invitrogen™ Supplier No. PA527378

Description

Rabbit Polyclonal Antibody

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, C2C12, Raw264.7, PC-12, HMGB1-transfected 293T. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Specifications

• Antigen: HMGB1
• Applications: Immunohistochemistry (Free Floating), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.43 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: Hmgb1
• Gene Accession No.: P09429, P12682, P63158, P63159
• Gene Alias: Ac2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product
• Gene Symbols: Hmgb1
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15289, 25459, 3146, 445521
• Target Species: Human, Mouse, Pig, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG