HMGN1/HMG14, p Ser20, p Ser24 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP178102

Catalog No. NBP178102

Description

HMGN1/HMG14 Polyclonal specifically detects HMGN1/HMG14 in Human, Sheep samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: HMGN1/HMG14 (p Ser20, p Ser24)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: FLJ31471, High mobility group nucleosome-binding domain-containing protein 1, high-mobility group (nonhistone chromosomal) protein 14, high-mobility group nucleosome binding 1, high-mobility group nucleosome binding domain 1, HMG14FLJ27265, MGC104230, MGC117425, non-histone chromosomal protein HMG-14, nonhistone chromosomal protein HMG-14
• Host Species: Rabbit
• Molecular Weight of Antigen: 10.66 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, Checkpoint signaling, Chromatin Research, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Histone Deacetylases, Phospho Specific, Stem Cell Markers
• Gene ID (Entrez): 3150
• Target Species: Human, Sheep, Chicken, Canine, Orangutan, Xenopus species
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Concentration: 1.2 mg/mL
• Dilution: Western Blot 1:500-1:2000, ELISA 1:5000-1:25000, Immunohistochemistry 20 ug/ml, Immunohistochemistry-Paraffin 20 ug/ml
• Gene Accession No.: P05114
• Gene Symbols: HMGN1
• Immunogen: This affinity purified HMGN1/HMG14 [p Ser20, p Ser24] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-28 of human HMGN1/HMG141/HMG14 protein (see below). (Uniprot: P05114)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This affinity-purified antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions). The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human HMGN pS20/pS24 proteins and the antibody is specific for the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with HMGN proteins from Xenopus species, chicken, mouse, bovine, dog, orangutan, rat and swine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only