Invitrogen™ HMGN1 Monoclonal Antibody (SPM515)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA137941

Catalog No. 01-672-925

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates. HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of the higher order chromatin structure thereby facilitating access to the underlying DNA sequence. Clustering of architectural elements, such as HMG proteins and linker histone subtypes into distinct domains, may lead to structural and functional heterogeneity along the chromatin fiber. In addition, HMG-14 and HMG-17 have been identified as constitutive components of mouse oocyte and embryonic chromatin that establish a link between the structure of embryonic chromatin and the normal progression of embryonic development.

Specifications

• Antigen: HMGN1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: HMGN1
• Gene Alias: FLJ27265; FLJ31471; high mobility group nucleosomal binding domain 1; high mobility group nucleosome binding domain 1; high mobility group nucleosome-binding domain-containing protein 1; high mobility group protein 14; high-mobility group (nonhistone chromosomal) protein 14; high-mobility group nucleosome binding 1; high-mobility group nucleosome binding domain 1; high-mobility group nucleosome-binding domain-containing protein 1; HMG 14; Hmg14; HMG-14; HMGN 1; Hmgn1; MGC104230; MGC117425; Non-histone chromosomal protein HMG-14; nonhistone chromosomal protein HMG-14
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 3150
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Clone: SPM515
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P05114
• Gene Symbols: HMGN1
• Immunogen: Spleen cells of patient with hairy cell leukemia.
• Quantity: 1 mL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM