Invitrogen™ HMOX1 Monoclonal Antibody (HO-1-1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1112

Catalog No. PIMA1112

Description

This antibody detects an ~32 kDa protein corresponding to the apparent molecular mass of HO-1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine and dog origins. This antibody does not cross-react with HO-2. This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

Specifications

• Antigen: HMOX1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide
• Gene Accession No.: P06762, P09601, P14901, Q5E9F2
• Gene Symbols: HMOX1
• Immunogen: Synthetic peptide corresponding to the sequence near the N-terminus (amino acids 1-30) of human Heme Oxygenase 1.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Bovine
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: HO-1-1
• Conjugate: Unconjugated
• Gene: HMOX1
• Gene Alias: bK286B10; D8Wsu38e; heat shock protein 32; heat shock protein, 32-kD; heme orygenase 1; heme oxygenase; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; heme oxygenase 1; heme oxygenase-1; Hemox; hemoxygenase; Heox; HEOXG; Hmox; Hmox1; HMOX1D; HO; Ho1; HO-1; Hsp32; P32 protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 15368, 24451, 3162, 442987, 513221
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov