Invitrogen™ hnRNP F Monoclonal Antibody (1G11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA518024

Catalog No. PIMA518024

Description

For IHC applications, it is necessary to perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining. In Western blot applications, this antibody detects a band at ~53 kDa (hnRNP F, predicted 49.8 kDa) and 56 kDa (hnRNP H, predicted 53.9 kDa).

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Specifications

• Antigen: hnRNP F
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: P52597, Q9Z2X1
• Gene Symbols: Hnrnpf
• Immunogen: proprietary immunogen.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Frog
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1G11
• Conjugate: Unconjugated
• Gene: Hnrnpf
• Gene Alias: 4833420I20Rik; AA407306; heterogeneous nuclear ribonucleoprotein F; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed; hnRNP F; HnRNP F protein; hnRNP H; Hnrnpf; HNRNPH1; Hnrpf; mcs94 1; mcs94-1; Nucleolin-like protein mcs94-1; OK/SW-cl.23; ribonucleoprotein F; ROF
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3185, 98758
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid