Invitrogen™ hnRNP L Monoclonal Antibody (4D11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA546550

Catalog No. PIMA546550

Description

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Western blot detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: hnRNP L
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: F1LQ48, P14866, Q8R081
• Gene Symbols: HNRNPL
• Immunogen: Human hnRNP proteins, from HeLa cells, purified by affinity chromatography on ssDNA agarose.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Amphibian
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 4D11
• Conjugate: Unconjugated
• Gene: HNRNPL
• Gene Alias: C79783; D830027H13Rik; Fblim1; FLJ35509; Heterogeneous nuclear ribonucleoprotein L; heterogenous nuclear ribonucleoprotein L; hnRNP L; Hnrnpl; hnRNP-L; Hnrpl; P/OKcl.14
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 15388, 3191, 80846
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov