Tocris Bioscience™ Hoechst Janelia Fluor 526

Description

Fluorogenic, green-emitting DNA probe

Hoechst Janelia Fluor™ 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor™ 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor™ 526 exhibits a quantum yield = 0.04. Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor™ 526 is suitable for multicolor microscopy experiments and for use in live cell imaging. Excitation maximum = 531 nm; emission maximum = 549 nm. To measure the absorbance spectrum of Hoechst Janelia Fluor™ 526 we recommend the following solvent: TFE plus 0.1% TFA.

Specifications

• Chemical Name or Material: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3 H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1 H,3 'H-[2,5 '-bibenzo[d]imidazol]-2 '-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)be
• Quantity: 100 μg
• Target: Fluorescent DNA Stains
• Molecular Formula: C₆₂H₅₈F₆N₁₀O₈0.4 CF₃CO₂H
• Purity: 0.95