Invitrogen™ HP1 alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120178

Catalog No. PIPA5120178

Description

Positive test controls include: HeLa, Jurkat, SH-SY5Y, A-549, MCF7, Mouse brain, 293T. The target is usually found in the following locations: Chromosome, Nucleus, centromere. Immunogen sequence: MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD.

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

Specifications

• Antigen: HP1 alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cbx5
• Gene Alias: 2610029O15Rik; antigen p25; C75991; Cbx5; Cbx5 antibody; chromobox 5; chromobox homolog 5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); chromobox protein homolog 5; chromobox-like protein 5; epididymis luminal protein 25; HEL25; heterochromatin protein 1 alpha; heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP1; HP1 alpha; HP-1 alpha antibody; HP1 alpha homolog; Hp1a; Hp1alpha; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; mHP1(alpha)
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12419, 23468
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 1.12 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P45973, Q61686
• Gene Symbols: Cbx5
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human HP1 alpha/HP1 alpha/CBX5 (NP_0011207941).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG