Invitrogen™ HPK1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA515184

Catalog No. PIPA515184

Description

HPK1 Polyclonal Antibody for Western Blot, IHC (P)

MAP4K1 (also known as HPK1) or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. MAP4K1 is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. MAP4K1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76. The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1.

Specifications

• Antigen: HPK1
• Classification: Polyclonal
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: Q92918
• Gene Symbols: MAP4K1
• Immunogen: KLH conjugated synthetic peptide between 321-350 amino acids from the C-terminal region of human MAP4K1 (HPK1).
• Quantity: 400 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Gene: MAP4K1
• Gene Alias: EC 2.7.11.1; Hematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK; HPK1; MAP4K1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; mHPK1; mitogen activated protein kinase kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase kinase 1
• Host Species: Rabbit
• Purification Method: Ammonium Sulfate Precipitation, Size-exclusion - Dialysis
• Regulatory Status: RUO
• Gene ID (Entrez): 11184
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid