MilliporeSigma™ HRD1, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS1623

Catalog No. ABS1623MI

Description

E3 ubiquitin-protein ligase synoviolin (UniProt Q86TM6; also known as Synovial apoptosis inhibitor 1) is encoded by the SYVN1 (also known as HRD1, KIAA1810) gene (Gene ID 84447) in human. E3 ubiquitin-protein ligase synoviolin is a multi-transmembrane ER membrane protein. Originally named Hrd1, synoviolin adopted the current name due to its upregulation in synovial fibroblasts from patients with rheumatoid arthritis as a result of exposure to inflammatory cytokines. Synoviolin plays a protective role against ER stress-induced apoptosis by mediating not only the degradation of ER misfolded proteins, but also the degradation of proapoptotic protein p53 as well as IRE1α (inositol-requiring enzyme 1α), a critical kinase regulating ER stress-induced cell death. Synoviolin is produced with a signal peptide (a.a. 1-22) sequence, the removal of which yields the mature 5-transmembrane (a.a. 42-62, 99-119, 141-161, 170-190, 225-245) protein, having its N-terminal end in the ER lumen (a.a. 23-41) and C-terminal end (a.a. 246-617) in the cytoplasm. Its C-terminal cytoplasmic end contains the p53/TP53-interacting region (a.a. 236-270), an atypical RING-type zinc finger domain (a.a. 291-330), and a proline-rich region (a.a. 339-478).

Specifications

• Antigen: HRD1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SYVN1, HRD1, KIAA1810
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Immunogen sequence is present in all three spliced isoforms of human HRD1/SYVN1 reported by UniProt (Q86TM6).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 155mM NaCl, 50mM Tris-HCl pH 7.4 with 0.05% Sodium Azide and 10% glycerol.
• Gene Accession No.: Q86TM6
• Immunogen: Linear peptide corresponding to the C-terminal sequence of human HRD1.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NP_115807
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.