Invitrogen™ HSC70 Monoclonal Antibody (13D3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3014

Catalog No. MA3014

Description

MA3-014 detects heat shock cognate protein 70 kDa (HSC70) from human, feline, primate, rat, and mouse tissues. This antibody displays slight cross-reactivity to HSP70. MA3-014 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation procedures By Western blot, this antibody detects a 70 kDa protein representing HSC70. By 2D gel electrophoresis, MA3-014 was found to bind strongly to HSC70 and faintly to HSP70 both before and after heat shock. Immunohistochemical staining of HSC70 in mouse spermatids/spermatozoa with MA3-014 results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa. The MA3-014 antigen is mouse spermatogenic cell protein.

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

Specifications

• Antigen: HSC70
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P11142, P63017, P63018
• Gene Symbols: Hspa8
• Immunogen: Mouse spermatogenic cell protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey, Feline
• Product Type: Antibody
• Isotype: IgM

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 13D3
• Conjugate: Unconjugated
• Gene: Hspa8
• Gene Alias: 2410008N15Rik; AAG2; constitutive heat shock protein 70; constitutively expressed heat shock protein; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate; heat shock cognate 70; heat shock cognate 71 kD protein; heat shock cognate 71 kDa protein; heat shock cognate 71 kDa protein-like protein; heat shock cognate 71-kd protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 70 cognate; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; heat shock protein family A (Hsp70) member 8; HEL-33; HEL-S-72p; HIP; HSC54; Hsc70; Hsc70 ATPase; HSC70 protein; Hsc71; Hsc73; HSP70; HSP71; HSP73; Hspa10; Hspa8; hspa8 protein; I79_022974; LAP; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; similar to 7O KDa heat shock cognate protein; SNC6; unnamed protein product; wu:fb01g06; wu:fi48b06
• Host Species: Mouse
• Purification Method: Affinity chromatography - MBP
• Regulatory Status: RUO
• Gene ID (Entrez): 101087422, 15481, 24468, 3312
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov