Invitrogen™ HSC70 Monoclonal Antibody (1F2-H5), APC

Catalog No.	Quantity
PIMA545189	200 μg

Catalog No. PIMA545189 Supplier Invitrogen™ Supplier No. MA545189

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-45189 was sufficient for detection of HSC70 in 10 μg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 73kDa. Does not cross react with HSP70.

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

Specifications

• Antigen: HSC70
• Applications: ELISA, Functional Assay, Immunohistochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot, Proximity Ligation Assay, Immunocytochemistry, Microarray
• Classification: Monoclonal
• Clone: 1F2-H5
• Concentration: 1 mg/mL
• Conjugate: APC
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene: Hspa8
• Gene Accession No.: P11142, P63017, P63018
• Gene Alias: 2410008N15Rik; AAG2; ARABIDOPSIS THALIANA HEAT SHOCK COGNATE PROTEIN 70-1; AT-HSC70-1; AtHsp70-1; constitutive heat shock protein 70; constitutively expressed heat shock protein; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate; heat shock cognate 70; heat shock cognate 71 kD protein; heat shock cognate 71 kDa protein; heat shock cognate 71 kDa protein-like protein; heat shock cognate 71-kd protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock cognate protein 70-1; heat shock protein 70 cognate; HEAT SHOCK PROTEIN 70-1; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; heat shock protein family A (Hsp70) member 8; HEL-33; HEL-S-72p; HIP; HSC54; Hsc70; Hsc70 ATPase; HSC70 protein; Hsc71; Hsc73; HSP70; HSP70-1; HSP71; HSP73; Hspa10; Hspa8; hspa8 protein; I79_022974; LAP; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; similar to 7O KDa heat shock cognate protein; SNC6; T22P11.90; T22P11_90; unnamed protein product; wu:fb01g06; wu:fi48b06
• Gene Symbols: Hspa8
• Host Species: Mouse
• Immunogen: Full length human HSC70.
• Purification Method: Protein G
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15481, 24468, 3312
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ