Invitrogen™ HSF1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA589420

Catalog No. PIPA589420

Description

Immunogen sequence: DARGHTDTEG RPPSPPPTST PEKCLSVACL DKNELSDHLD AMDSNLDNLQ TMLSSHGFSV DTSALLDLFS PSVTVPDMSL PDLDSSLASI QELLSPQEPP RPPEAENSSP DSGKQLVHYT AQPLFLLDPG SVDTGSNDLP VLFELGEGSY FSEGDGFAED PTISLLTGSE PPKAKDPTVS; Positive Samples: HeLa, K-562, HepG2, MCF7; Cellular Location: Cytoplasm, Nucleus.

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

Specifications

• Antigen: HSF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Hsf1
• Gene Alias: AA960185; heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF; HSF 1; Hsf1; Hsf1alpha; Hsf1beta; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; HSTF1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15499, 3297, 79245
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.83 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: P38532, Q00613
• Gene Symbols: Hsf1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_0055171).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG